Human Anti-C3b Recombinant Antibody (clone S77) (CAT#: PABL-420)
Recombinant Human Antibody (S77) is capable of binding to C3b, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-C3b mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-C3b proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody blocks binding of factor B to C3b inhibiting the first step in the formation of the alternative pathway C3 convertase.
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FuncS
Figure 1 Generation of a phage antibody that selectively binds to C3b, but not native C3.
Binding of S77 IgG to C3b. Binding is expressed as the ratio of optical densities of the reaction mixtures measured at 450 and 630 nm.
Katschke, K. J., Stawicki, S., Yin, J., Steffek, M., Xi, H., Sturgeon, L., ... & van Lookeren Campagne, M. (2009). Structural and functional analysis of a C3b-specific antibody that selectively inhibits the alternative pathway of complement. Journal of Biological Chemistry, 284(16), 10473-10479.
Inhib
Figure 2 S77 inhibits fH and sCR1 binding to C3 and inhibits fH and sCR1co-factor activity.
Microtiter plates were coated with C3b, and fH wasadded in the presence of increasing concentrations of S77 or control Fab. Binding of fH to C3b was determined using an anti-fH antibody and a secondary HRPO-conjugated antibody. Absorbance of the reaction mixture was measured at 450 nm.
Katschke, K. J., Stawicki, S., Yin, J., Steffek, M., Xi, H., Sturgeon, L., ... & van Lookeren Campagne, M. (2009). Structural and functional analysis of a C3b-specific antibody that selectively inhibits the alternative pathway of complement. Journal of Biological Chemistry, 284(16), 10473-10479.
IP
Figure 3 Cofactor activity for fI-mediated cleavage of C3b was measured by incubating C3b and fI with fH or increasing concentrations of S77 or control Fab.
The mixture was incubated at 37 °C, and the samples were analyzed by gel-electrophoresis and Simply Blue staining.
Katschke, K. J., Stawicki, S., Yin, J., Steffek, M., Xi, H., Sturgeon, L., ... & van Lookeren Campagne, M. (2009). Structural and functional analysis of a C3b-specific antibody that selectively inhibits the alternative pathway of complement. Journal of Biological Chemistry, 284(16), 10473-10479.
Inhib
Figure 4 S77 inhibits the alternative, but not classical, pathway C5 convertase in human serum.
S77 does not inhibit CP convertase activation. IgM-coated sheep erythrocytes were incubated with factor B-depleted human serum and increasing concentrations of S77 or anti-C5 antibody.
Katschke, K. J., Stawicki, S., Yin, J., Steffek, M., Xi, H., Sturgeon, L., ... & van Lookeren Campagne, M. (2009). Structural and functional analysis of a C3b-specific antibody that selectively inhibits the alternative pathway of complement. Journal of Biological Chemistry, 284(16), 10473-10479.
ELISA
Figure 5 S77 inhibits the alternative pathway C3 and C5 convertase.
C3 and factors B and D were incubated in the presence of increasing concentrations of S77 or control Fab. The concentration of C3a des-Arg reaction product was determined by ELISA.
Katschke, K. J., Stawicki, S., Yin, J., Steffek, M., Xi, H., Sturgeon, L., ... & van Lookeren Campagne, M. (2009). Structural and functional analysis of a C3b-specific antibody that selectively inhibits the alternative pathway of complement. Journal of Biological Chemistry, 284(16), 10473-10479.
Specifications
- Immunogen
- Human complement component 3
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG
- Specificity
- C3b
- Species Reactivity
- Human
- Clone
- S77
- Applications
- FuncS, Inhib, IP, ELISA
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-Linked Immunosorbent Assay, Inhibition, Immunoprecipitation and Function Assay.
Target
- Alternative Names
- C3; complement component 3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p; complement C3; prepro-C3; C3a anaphylatoxin; complement component C3; complement component C3a; complement component C3b; acylation-stimulating protein cleavage product; epididymis secretory sperm binding protein Li 62p; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1;
- Gene ID
- 718
- UniProt ID
- P01024
Related Resources
Systemic Lupus Erythematosus
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Antibody Products
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ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-417 | Human Anti-C3b Recombinant Antibody (clone S77); Fab Fragment | ELISA | Human Fab |
| HPAB-M0449-YC-F(E) | Mouse Anti-C3b Recombinant Antibody (clone AC3b); Fab Fragment | ELISA, Inhib | Mouse Fab |
| HPAB-M0450-YC-F(E) | Human Anti-C3b Recombinant Antibody (clone C3b); Fab Fragment | ELISA, Inhib | Humanized Fab |
| HPAB-M0451-YC-F(E) | Human Anti-C3b Recombinant Antibody (clone hAC3b); Fab Fragment | ELISA, Inhib | Humanized Fab |
| HPAB-0954WJ-F(E) | Human Anti-C3b Recombinant Antibody (clone MOR09556); Fab Fragment | ELISA | Human Fab |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-420, RRID: AB_3111656)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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